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recent preprint articles

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Recent Preprint Articles

Donahue EFK, Hepowit NL, Keuchel B, Mulligan AG, Johnson DJ, Ellisman M, Arrojo E Drigo R, MacGurn JA, Burkewitz K. ER-phagy drives age-onset remodeling of endoplasmic reticulum structure-function and lifespan. bioRxiv. 2024. doi: 10.1101/2024.08.07.607085.

Hepowit NL, Singkhek HL, Frazier KD, Johnson DJ, MacGurn JA. Methionine-triggered growth arrest reveals activation of Gcn2 by methionine transporter endocytosis. bioRxiv. 2025. doi: 10.1101/2025.05.12.653625.

Ebert AC, Hepowit NL, Martinez TA, Vollmer H, Singkhek HL, Frazier KD, Kantejeva SA, Patel MR, MacGurn JA. Sphingolipid metabolism drives mitochondria remodeling during aging and oxidative stress. bioRxiv. 2025. doi: 10.1101/2025.02.26.640157.

Published Manuscripts

Romero-Fernandez W, Carvajal-Tapia C, Prusky A, Katdare KA, Wang E, Shostak A, Ventura-Antunes L, Harmsen HJ, Lippmann ES, Fuxe K, MacGurn JA, Borroto-Escuela DO, Schrag MS. Detection, visualization and quantification of protein complexes in human Alzheimer’s disease brains using proximity ligation assay. Sci Rep. 2023 Jul 24;13(1):11948. doi: 10.1038/s41598-023-38000-4. PMID: 37488165.

Hepowit NL, Moon B, Ebert AC, Dickson RC*, MacGurn JA*. Art2 mediates selective endocytosis of methionine transporters during adaptation to sphingolipid depletion. J Cell Sci. 2023 Jul 15;136(14):jcs260675. doi: 10.1242/jcs.260675. Epub 2023 Jul 25. PMID: 37337792.
[* indicates co-corresponding authors]

Qualls-Histed SJ, Nielsen CP, MacGurn JA. Lysosomal trafficking of the glucose transporter GLUT1 requires sequential regulation by TXNIP and ubiquitin. iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. eCollection 2023 Mar 17. PMID: 36890792.

Hepowit NL, Blalock E, Lee S, Bretland KM, MacGurn JA*, Dickson RC*. Reduced sphingolipid biosynthesis modulates proteostasis networks to enhance longevity. Aging. 2023 Jan 14;15. doi: 10.18632/aging.204485. PMID: 36640272.
[* indicates co-corresponding authors]

Hepowit NL, Denise AS, MacGurn JA. Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast. Methods Mol Biol. 2023; 2591:283-295. doi: 10.1007/978-1-0716-2803-4_17. PMID: 36350555.

Date SS, Xu P, Hepowit NL, Diab NS, Best J, Xie B, Du J, Strieter ER, Jackson LP, MacGurn JA, Graham TR. Ubiquitination drives COPI priming and Golgi SNARE localization. eLife. 2022 Jul 29;11:e80911. PMID: 35904239.

Hepowit NL, Macedo JK, Young LE, Liu K, Sun RC, MacGurn JA*, Dickson RC*. Enhancing lifespan of budding yeast by pharmacological lowering of amino acid pools. Aging. Mar 21;13(6):7846-7871. doi: 10.18632/aging.202849. Epub 2021 Mar 21. PMID: 33744865.
[* indicates co-corresponding authors]

Hepowit NL, Kolbe CC, Zelle SR, Latz E, MacGurn JA. Regulation of ubiquitin and ubiquitin-like modifiers by phosphorylation. FEBS J. 2021 Jul 2. doi: 10.1111/febs.16101. PMID: 34214249

Hepowit NL, Pereira KN, Tumolo JM, Chazin WJ, MacGurn JA. Identification of ubiquitin Ser57 kinases regulating the oxidative stress response in yeast. eLife. 2020 Oct 19;9:e58155. doi: 10.7554/eLife.58155. PMID: 33074099.

Nielsen CP and MacGurn JA. Coupling conjugation and deconjugation activities to achieve cellular ubiquitin dynamics. Trends Biochem Sci. 2020 May; 45(5):427-439. doi: 10.1016/j.tibs.2020.01.008. PMID: 32311336.

Wong S, Hepowit NL, Port SA, Yau RG, Peng Y, Azad N, Habib A, Harpaz N, Schuldiner M, Hughson FM, MacGurn JA, Weisman LS. Cargo release from myosin V requires the convergence of parallel pathways that phosphorylate and ubiquitylate the cargo adaptor. Curr Biol. 2020 Sep 3: S0960-9822(20)31252-5. doi: 10.1016/j.cub.2020.08.062. PMID: 32916113

Strunk BS, Steinfeld N, Lee S, Natsuko J, Muñoz-Rivera C, Meeks G, Thomas A, Mapp AK, MacGurn JA, Weisman LS. Roles for a lipid phosphatase in the activation of its opposing lipid kinase. Mol Biol Cell. 2020 Aug 1; 31(17): 1835-1845. doi: 10.1091/mbc.E18-09-0556. PMID: 32583743.

Tumolo JM, Hepowit NL, Joshi SS, MacGurn JA. A Snf1-related nutrient-responsive kinase antagonizes endocytosis in yeast. PLoS Genet. 2020 Mar 19;16(3):e1008677. doi: 10.1371/journal.pgen.1008677. PMID: 32191698.

Baile MG, Guiney EL, Sanford EJ, MacGurn JA, Smolka MB, Emr SD. Activity of a ubiquitin ligase adaptor is regulated by disordered insertions in its arrestin domain. Mol Biol Cell. 2019 Oct 16: mbcE19080451. doi: 10.1091/mbc.E19-08-0451. PMID: 31618110.

Nielsen CP, Jernigan KK, Diggins NL, Webb DJ, MacGurn JA. USP9X deubiquitylates DVL2 to regulate WNT pathway specification. Cell Reports. 2019 Jul 23; 28(4):1074-1089.e5. doi: 10.1016/j.celrep.2019.06.083.

Lee S, Ho HC, Tumolo JM, Hsu PC, MacGurn JA. Methionine triggers Ppz-mediated dephosphorylation of Art1 to promote cargo-specific endocytosis. J Cell Biol. 2019 Jan 4. jcb.201712144.

Lee S, Tumolo JM, Ehlinger AC, Jernigan KK, Qualls-Histed SJ, Hsu PC, McDonald WH, Chazin WJ, MacGurn JA. Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin. eLife. 2017 Nov 13;6:e29176. PMCID: PMC5706963.

Xu P, Hankins HM, MacDonald C, Erlinger SJ, Frazier MN, Diab NS, Piper RC, Jackson LP, MacGurn JA, Graham TR. COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. eLife. 2017 Nov 13;6:e29176. PMCID: PMC5706963.

Ho HC, MacGurn JA, Emr SD. Deubiquitinating enzymes Ubp2 and Ubp15 regulate endocytosis by limiting ubiquitination and degradation of ARTs. Mol Biol Cell. 2017 May 1; 28(9): 1271-1283. doi: 10.1091/mbc.E17-01-0008. PMID: 28298493.

MacGurn JA. Garbage on, garbage off: New insights into plasma membrane protein quality control. Current Opinion in Cell Biology. 2014 Aug; 29: 92-8. PMCID: PMC4130900.

Zhao Y*, MacGurn JA*, Liu M, Emr SD. The ART-Rsp5 ubiquitin ligase network comprises a plasma membrane quality control system that protects yeast cells from proteotoxic stress. eLife. 2013 Apr 16;2:e00459. PMCID: PMC3628405.

Manford AG, Stefan CJ, Yuan HL, MacGurn JA, Emr SD. ER-to-plasma membrane tethering proteins regulate cell signaling and ER morphology. Dev Cell. 2012 Dec 11; 23(6): 1129-40.

Ling Y, Stefan CJ, MacGurn JA, Audhya A, Emr SD. The dual PH domain protein Opy1 functions as a sensor and modulator of PtdIns(4,5)P(2) synthesis. EMBO J. 2012 May 4; 31(13): 2882-94.

MacGurn JA, Hsu PC, Emr SD. Ubiquitin and Membrane Protein Turnover: from Cradle to Grave. Annu Rev Biochem. 2012 Jul 7; 81:231-59.

MacGurn JA*, Hsu PC*, Smolka MS, Emr SD. TORC1 Regulates Endocytosis via Npr1-mediated Phosphoinhibition of a Ubiquitin Ligase Adaptor. Cell. 2011 Nov 23; 147(5): 1104-17.

Lin CH*, MacGurn JA*, Chu T, Stefan CJ, Emr SD. Arrestin-related ubiquitin-ligase adaptors regulate Endocytosis and protein turnover at the cell surface. Cell. 2008 Nov 14; 135(4): 714-25.

McLaughlin B, Chon JS, MacGurn JA, Carlsson F, Cheng TL, Cox JS, Brown EJ. A mycobacterium ESX-1-secreted virulence factor with unique requirements for export. PLoS Pathog. 2007 Aug 3; 3(8): e105.

Greenstein AE, MacGurn JA, Baer CE, Falick AM, Cox JS, Alber T. M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. PLoS Pathog. 2007 Apr; 3(4): e49.

MacGurn JA and Cox JS. A genetic screen for Mycobacterium tuberculosis mutants defective for phagosome maturation arrest identifies components of the ESX-1 secretion system. Infect Immun. 2007 Jun; 75(6): 2668-78.

MacGurn JA, Raghavan S, Stanley SA, Cox JS. A non-RD1 gene cluster is required for Snm secretion in Mycobacterium tuberculosis. Mol Microbiol. 2005 Sep; 57(6): 1653-63.